玉米皮汽爆–水热提取阿魏酰阿拉伯低聚木糖工艺研究

Steam Explosion – Hydrothermal Extraction Process of Feruloylated Arabinoxylan Oligosaccharides from Corn Husk

摘要:

为实现玉米皮的高值化利用,本研究采用蒸汽爆破辅助微波水热工艺提取阿魏酰阿拉伯低聚木糖(F-AXOs),以糖浓度、糖得率、聚合度为评价指标筛选工艺参数,并探究产物的抗氧化与益生元活性。结果表明,低压短时汽爆预处理可显著促进F-AXOs生成,提高低聚糖得率,在1.5MPa、2 min的汽爆条件结合水热处理下,F-AXOs浓度高达11.56mmol/L,低聚糖总得率达76.11%。机理分析显示,汽爆预处理有效破坏了细胞壁中半纤维素结构,促使无定形区组分降解,从而强化低聚糖溶出。功能评价表明,汽爆-水热产物对1,1-二苯基-2-三硝基苯肼(DPPH)及2,2′-联氮双(3-乙基苯并噻唑啉-6-磺酸)阳离子(ABTS+)自由基的清除能力显著优于低聚木糖(XOS)对照,表现出较强的抗氧化活性;体外发酵过程中短链脂肪酸(SCFAs)生成速率较快,证实该提取物具有良好的益生元特性。综上,汽爆-水热工艺在无需额外添加化学试剂的条件下有效提升了F-AXOs浓度,所得产物表现出良好的抗氧化与益生元活性,为其在食品领域的潜在应用提供了理论依据。

Abstract:

Aiming to achieve high-value utilization of corn husk, a steam explosion-assisted microwave hydrothermal process was employed to extract feruloylated arabinoxylan oligosaccharides (F-AXOs). Process parameters were screened by using sugar concentration, sugar yield, and degree of polymerization as evaluation criteria, and the antioxidant and prebiotic activities of the products were investigated. The results indicated that short-duration, low-pressure steam explosion pretreatment significantly promoted F- AXOs formation and increased oligosaccharide yield. Under steam explosion conditions of 1.5MPa for 2min combined with hydrothermal treatment, the F-AXOs concentration peaked at 11.56mmol/ L, and the total oligosaccharide yield reached 76.11%. Mechanistic analysis revealed that steam explosion pretreatment effectively disrupted the hemicellulose structure in the cell wall, promoting the degradation of the amorphous region, thereby enhancing oligosaccharide elution. Functional evaluation indicated that the steam explosion hydrothermal products exhibited antioxidant activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2, 2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) cation (ABTS?) radicals, demonstrating strong antioxidant activity. The rapid rate of short-chain fatty acids (SCFAs) production during in vitro fermentation confirmed that this extract possessed excellent prebiotic properties. In summary, the steam explosion-hydrothermal process effectively increased the concentration of F-AXOs without the need for additional chemical reagents. The obtained products exhibited good antioxidant and prebiotic activity, providing a theoretical basis for their potential applications in the food industry.