Abstract:Above-ground biomass (AGB) is one of the most important indicators for crop growth assessment and yield prediction. To establish the rapeseed AGB estimation model based on UAV multispectral data, a UAV equipped with a 6-band multispectral sensor was utilized to acquire remote sensing images during the seedling, elongation, flowering, and pod stages of rapeseed. The Fourier transform was conducted to UAV multispectral images, and frequency-domain features were extracted based on Fourier spectrum. Then, the correlation between the frequency-domain features and AGB was analyzed at different growth stages, and the frequency components representing the spatial distribution of organs (leaves, flowers, and pods) were extracted and fused with spectral features. Three machine learning algorithms of back propagation (BP) neural network, random forest (RF), and eXtreme gradient boosting (XGBoost) were employed to establish AGB estimation models for different growth stages and the accuracy of AGB estimation with different input variables was compared. The results indicated that the fusion of spectral features and organ spatial distribution features can improve the accuracy of AGB estimation model. Among the three machine learning algorithms, BP demonstrated more stable and better AGB fitting performance with the fused features. Using the reflectance of the six bands and specific frequency-domain features (low-frequency components for the leaf and pod stages;high-frequency components for the flowering stage;weighted reconstruction of low and high frequency components for the entire growth stage) as input variables, BP achieved accurate estimation of rapeseed AGB across different growth stages. The test set results were as follows, coefficient of determination (R2) of leaf stage was 0.87 with root mean square error (RMSE) of 178.44 g/m2, R2 of flowering stage was 0.66 with RMSE of 357.76 g/m2, R2 of pod stage was 0.87 with RMSE of 258.71 g/m2 and R2 of entire growth period was 0.79 with RMSE of 388.90 g/m2. The results can provide technical support for the precise and efficient acquisition of rapeseed AGB at a regional scale.