Abstract:Slightly acidic electrolyzed water is a kind of bactericidal preparation with pH value slightly lower than 7.0 by electrolyzing dilute hydrochloric acid solution or electrolyzing low concentration dilute hydrochloric acid and electrolyte (NaCl/KCl) under nondiaphragm condition. It has a strong ability to kill pathogenic bacteria, but there is little research on the ability and mechanism of killing fungal spores by slightly acidic electrolyzed water. The killing effect of slightly acidic electrolytic water on pure culture spore suspension and in situ culture of Botrytis cinerea was studied, and the germicidal mechanism of water on Botrytis cinerea was preliminarily studied by scanning electron microscopy and transmission electron microscopy. The results showed that the bactericidal ability of slightly acidic electrolyzed water to Botrytis cinerea was increased significantly with the increase of sterilization time and available chlorine concentration (ACC), and bactericidal rate can achieve 99.99% when the available chlorine concentration was not less than 30mg/L and kill time was 10min. The growth state of Botrytis cinerea colony in situ after treatment with slightly acidic electrolytic water showed that slightly acidic electrolytic water could inhibit colony expansion, and the higher the concentration was, the stronger the inhibition effect was. After 3d of treatment with slightly acidic electrolytic water with 30mg/L available chlorine, the mycelium gradually turned yellow and withered until death, and the colony was stopped. It was suggested that microacid electrolyzed water can kill the new spores of Botrytis cinerea and inhibit the growth of mycelia. Through microscope observation of scanning and transmission electron, it was found that treatment with acidic electrolyzed water can make separation of membrane and cell wall, cytoplasmic overflow and abnormal lysis of organelles. It was speculated that these may be the reasons which can achieve the bactericidal effect.
