Extraction Technology and Antioxidant Activities of Bioactive Components from Terminalia chebula Retz.

Abstract:

The extraction technology and antioxidant activities of the bioactive components from Terminalia chebula Retz. were studied. The results showed that the optimal parameters of the extraction technology were that the concentration of ethanol was 50%, the ratio of extraction solution to sample was 20, the extraction temperature was 60℃, the extraction times was 4 and the extraction time was 7min. The crude extracts from this plant were proved to have strong antioxidant activities through the results of the reducing power, the FRAP assay and the DPPH radicals scavenging percentages. The bioactive antioxidants of Terminalia chebula Retz. were mainly laid in the ethyl acetate fraction based on the bioassay-guided method. The OD values at 700 nm (reducing power)，FRAP values (OD593nm) and DPPH radicals-scavenging percentages of this fraction with the concentrations of 25 and 50μg/mL were 0.197±0.002 and 0.380±0.006, 0.272±0.023 and 0.631±0.002, (86.838±0.600) % and (90.318±0.917)%, respectively. The correlation showed that the strong antioxidant activity mainly resulted from the high contents of total phenols and total flavonoids.