Abstract:Aiming to improve the inversion accuracy of single-plant dry matter in relay-planted maize in arid regions and support precision irrigation decision-making, focusing on the insufficient stability of prediction models under small-sample conditions.Taking relay-planted maize in southern Xinjiang as the research object, single-plant dry matter prediction was conducted based on UAV multispectral imagery.Canopy images were acquired at the silking, grain-filling, and maturity stages, and vegetation indices, including NDVI, GNDVI, NDRE, LCI, and OSAVI were extracted.Meanwhile, structural variables such as plant height and stem diameter measured synchronously in the field were incorporated to construct spectral-structural fusion prediction models.Under small-sample conditions, ridge regression, random forest, and an ensemble model were used for modeling, and model accuracy and stability under different variable combinations and growth stages were systematically analyzed.The results showed that all vegetation indices were significantly positively correlated with single-plant dry matter, with NDRE and LCI performing best and the strongest correlations observed at the grain-filling stage.Structural variables improved model accuracy and showed clear stage-specific effects: plant height was more important at the silking stage, whereas stem diameter contributed more stably at the grain-filling and maturity stages.The best prediction performance was obtained at the grain-filling stage.The validation R2 of the ridge model was increased from 0.71 to 0.90, while that of the ensemble model increased from 0.70 to 0.87, indicating a strong synergistic effect between spectral and structural information.At the single-plant scale, model accuracy depended strongly on growth stage, with performance decreasing in the order of grain-filling, silking, and maturity stages.These findings suggested that integrating red-edge-sensitive vegetation indices with structural variables can effectively improve dry matter prediction under small-sample conditions, with the grain-filling stage being the optimal modeling period.The research results can provide data support for precision irrigation zoning and water regulation in arid regions.