Abstract:Aiming to improve the efficiency and accuracy of non-destructive testing in pigeon egg breeding and incubation management, a hyperspectral transmission imaging recognition process was established for the rapid determination of the fertilization status of breeding pigeon eggs in the early stages of incubation. Original hyperspectral data were collected by using a hyperspectral transmission device, and after black-and-white correction, the entire egg was taken as the region of interest to obtain its average spectral information. Three preprocessing algorithms were applied: standard normal variate (SNV), wavelet transform (WT), and multiplicative scatter correction (MSC). Using a KS sample selection strategy, the data were split into training and test sets at a ratio of 7 ∶ 3. Feature wavelength intervals were selected by BiPLS, iPLS, and SiPLS for spectral interval screening. Based on this, linear models (LDA, LR )and nonlinear classification models (XGBoost )were trained, and the optimal preprocessing-feature selection-modeling combination and key discriminative wavelengths were analyzed. First derivative processing was used for a detailed analysis of the spectral differences between fertilized and unfertilized eggs. The results showed that, before incubation, the BiPLS-LDA-WT algorithm achieved the best classification performance, with a recall rate of 0. 98 for fertilized eggs, an accuracy of 0. 87, and an F1-score of 0. 89. On the 1st, 2nd, and 3rd days of incubation, the classification accuracies were 0. 92, 0. 95, and 1. 00, respectively, with recall rates of 0. 98, 1. 00, and 1. 00. The proposed integrated hyperspectral feature interval selection and multi-model comparison framework demonstrated good accuracy and interpretability, providing technical support for early non-destructive fertilization detection and incubation management of breeding pigeon eggs.