Abstract:Rapid extraction of crop row centerlines from drone aerial imagery is crucial for navigating agricultural tasks such as inter-row spraying, weeding, and harvesting. Focusing on cotton seedlings in Xinjiang’s field crops, an aerial image-based crop row extraction method was proposed by using intercept density clustering. Firstly, aerial images underwent green color feature extraction, maximum inter-class variance analysis, and image segmentation projection to obtain feature point sets for crop rows. Secondly, the feature point set underwent crop row clustering and segmentation by using an intercept-density-based clustering algorithm. Finally, the centerlines of each crop row were fitted by using the least squares method and projected back to the WGS-84 coordinate system, providing path information for GNSS-based inter-row navigation operations of agricultural machinery. Field experiments using high-precision RTK-GNSS receiver data as reference validated this large-scale cotton field crop row identification and positioning method. Results indicated that when applying segmentation projection methods with pixel spacing of 15 pixels, 45 pixels, 75 pixels, and 105 pixels to extract feature points for crop row centerline fitting, the maximum average lateral deviation values were 0.022m, 0.024m, 0.025m, and 0.112 m, respectively. The maximum standard deviations for lateral deviation were 0.027m, 0.028m, 0.028m, and 0.032m;the average angular deviations were 0.004°, 0.003°, 0.002°, and 0.005°, respectively, with standard deviations of 0.002°, 0.001°, 0.001°, and 0.002°;the time required for centerline extraction was 348.35s, 101.93s, 76.29s, and 63.33s. Considering both accuracy and efficiency, a segmentation interval of 75 pixels was the optimal choice. This method was suitable for large-scale crop row identification and positioning in cotton fields using drone aerial imagery, providing sufficient path information for mechanized field management and row-based navigation during cotton harvesting operations.