Abstract:Estimation of chlorophyll status at jointing stage of winter wheat is important for nutritional diagnosis of winter wheat. The UAV remote sensing platform was used to obtain the remote sensing information of winter wheat growth at the jointing stage, the multispectral vegetation indexes, RGB image texture features and coverage information were extracted, the models for estimating the winter wheat SPAD values were constructed based on the multivariate linear regression (MLR) and random forest regression (RFR). Then the effects of multispectral vegetation indexes, textural features, cover information, and combining them with each other on the estimation of winter wheat SPAD values were analyzed. The results showed that the combination of multispectral vegetation indexes, texture features, canopy coverage (combination of two or three types of parameters) can be used for the estimation of winter wheat SPAD values at the jointing stage, and the combination of more types of parameters improved the estimation accuracy of winter wheat SPAD values compared with the combination of single-type parameters or two types of parameters. And the accuracy of the winter wheat SPAD estimation model constructed based on the same parameters by using RFR was higher than that of the model constructed by MLR. Among them, the model constructed based on the three types of parameters had the highest estimation accuracy of winter wheat SPAD values, with R2 of 0.78 and RMSE of 2.08. Moreover, the effects of each type of parameters on the models accuracy improvement in descending order were multispectral vegetation indexes, texture features, and canopy coverage. Among them, the accuracy of the model constructed by multispectral vegetation indexes was similar to that of the model constructed by texture features. Canopy coverage had the smallest improvement in the estimation accuracy of SPAD values, but combining other features could improve the estimation accuracy of winter wheat SPAD values (R2 was increased by 0.02~0.03 for the RFR models). The combination of multispectral vegetation indexes, texture features and canopy coverage improved the accuracy of the models, providing a fast technical reference solution for winter wheat SPAD values estimation.