基于氮掺杂石墨烯量子点的便携式猪肉新鲜度实时荧光检测系统研究

Fluorescence Sensor Based on Nitrogen-doped Graphene Quantum Dot Nanomaterials for Pork Freshness Detection

摘要:

提出了一种新型的便携式实时荧光检测系统，旨在实现生鲜猪肉新鲜度的实时检测。该系统主要由两大部分组成：氮掺杂石墨烯量子点（N-GQDs）荧光纳米材料的研究和便携式荧光检测装置的创制。通过透射电子显微镜、傅里叶变换红外光谱和X射线光电子能谱等技术对N-GQDs形貌、结构和表面官能团进行分析，证实N-GQDs被成功合成；探讨了N-GQDs的光学特性，证明了N-GQDs对胺类物质具有优异的灵敏度和选择性；通过响应机理分析，表明N-GQDs与NH3之间产生了静态猝灭效应。便携式荧光传感器检测装置包括硬件设计和软件设计，主要包含5个模块，分别是光源激发模块、荧光检测模块、温湿度检测模块、无线通讯模块和阈值报警模块；通过无线通讯模块将检测数据实时传输至手机APP和上位机，实现了数据的可视化和存储。将N-GQDs荧光纳米材料与便携式荧光检测装置结合开发了便携式实时荧光猪肉新鲜度检测系统，并在生鲜猪肉中进行了系统性能实验验证。实现冷藏（4℃）和室温（25℃）条件下猪肉TVB-N含量精准检测并表现出优异的线性关系（R225℃=0.97，R24℃=0.92）。

Abstract:

A portable real-time fluorescence detection system was proposed for real-time detection of fresh pork freshness. The system consisted of two main parts, i.e., the research of nitrogen-doped graphene quantum dots (N-GQDs) fluorescent nanomaterials and the creation of portable fluorescence detection device. The morphology, structure and surface functional groups of N-GQDs were analyzed by transmission electron microscopy, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy, and it was confirmed that N-GQDs was successfully synthesized. The optical properties of N-GQDs were discussed, and it was proved that N-GQDs had excellent sensitivity and selectivity to amines. The response mechanism analysis showed that there was a static quenching effect between N-GQDs and NH3. The portable fluorescence sensor detection device included hardware design and software design, which mainly included five modules, namely light source excitation module, fluorescence detection module, temperature and humidity detection module, wireless communication module and threshold alarm module. Through the wireless communication module, the detection data was transmitted to the mobile phone APP and the host computer in real time, and the data was visualized and stored. A portable real-time fluorescence pork freshness detection system was developed by combining N-GQDs fluorescent nanomaterials with portable fluorescence detection device, and its performance was verified by experiments in fresh pork. The precise detection of TVB-N in pork at cold storage (4℃) and room temperature (25℃) showed excellent linear relationships (R225℃=0.97, R24℃=0.92).