不同空化射流条件下大豆蛋白-白藜芦醇互作结合机制与抗氧化活性研究

Effect of Cavitation Jet on Interaction and Antioxidant Activity of Soybean Protein-Resveratrol

摘要:

为探究空化射流预处理对大豆分离蛋白-白藜芦醇（SPI-RES）复合物的影响，对SPI进行空化射流预处理（0、2、4、6、8、10min）后，与RES非共价结合形成复合物。通过荷载量和包埋率研究了SPI对RES的结合情况，采用内源荧光光谱、傅里叶红外光谱及分子对接技术研究了SPI和RES之间的相互作用机制，通过粒径、ζ-电位、表面疏水性、抗氧化活性等考察了复合物的物理化学性质和功能特性。结果表明：经过一定时间的空化射流处理后，SPI对RES包埋率和荷载量显著增加，复合物粒径和ζ-电位分别减小和增大。内源荧光光谱表明RES对SPI的淬灭为静态淬灭，反应是自发进行的。傅里叶红外光谱表明适当的空化射流预处理促进了SPI从有序结构转变为无序结构，从而结合更多的RES。热力学参数和分子对接结果表明疏水相互作用是主要作用力，还涉及氢键。此外，适当的空化射流预处理后，SPI-RES复合物表面疏水性及抗氧化活性均有所增加。本研究为空化射流预处理大豆分离蛋白应用领域的开拓和脂溶性活性物质保健食品的开发提供了前期理论基础。

Abstract:

To investigate the effect of cavitation jet pretreatment on soybean protein isolate-resveratrol (SPI-RES) complexes, SPI was subjected to cavitation jet pretreatment (0min, 2min, 4min, 6min, 8min and 10min) before non-covalent binding with RES to form complexes. The binding of SPI to RES was studied through loading amount and encapsulation efficacy. The interaction and binding mechanism between SPI and RES were studied by using fluorescence spectroscopy, Fourier transform infrared spectroscopy, and molecular docking techniques. In addition, the physical and chemical properties and functional characteristics of the SPI-RES complexes were investigated, including ζ-potential, surface hydrophobicity, and antioxidant activity. The results indicated that after a certain period of cavitation jet pretreatment, SPI significantly increased the encapsulation efficacy and loading amount of RES, and the particle size and ζ-potential of complexes was decreased and increased respectively. The fluorescence spectrum indicated that the quenching of SPI by RES was static quenching, and the reaction was spontaneous. Fourier transform infrared spectroscopy indicated that appropriate cavitation jet pretreatment promoted the transition of SPI from ordered to disordered structures, thereby combining more RES. The thermodynamic parameters and molecular docking results indicated that hydrophobic interactions were the main forces and involved hydrogen bonds. In addition, after appropriate cavitation jet pretreatment, the surface hydrophobicity and antioxidant activity of SPI-RES complexes were increased. The research result can provide a preliminary theoretical basis for the exploration of the application field of cavitation jet pretreatment of soybean protein isolate and the development of fat-soluble active substances in health food.