Abstract:In order to realize the non-destructive and automatic measurement of factory plug seedlings, a non-destructive monitoring method based on Kinect camera was proposed. With the cucumber plug seedlings, the Kinect camera was set up directly above the plug seedlings to obtain the depth image and the color image, and pixels matching was carried between obtained two kind of images. The germination rate was monitored by preprocessing, threshold segmentation, morphological operations and connected component statistics on color image. The center pixel point and the corresponding depth of the leaf were calculated by using the seedling contour obtained by image segmentation, and depth map, so as to obtain the height from the camera to the center of leaf. Lastly, combined with the distance of the camera to the plug tray, the seedling height of plug tray was monitored. The valid depth image was obtained by an algorithm which combined straight-through filtering, conditional filtering, and boundary-preserving filtering to effectively remove background noise around the plug seedlings and depth data with large fluctuations. Then, the leaf area was calculated by blade reconstruction. Based on the results of the height and leaf area of the plug seedling, the estimation of the healthy index was put forward. The method was verified by monitoring the growth process of plug seedlings. Within five days after germination, the monitored germination rate error was no more than 1.567%. The goodness of fit R 2 between the monitored plant height and the actual plant height was 0.875, and the RMSE was 1.395mm. The average error of the calculated leaf area was 2.15%, and the R 2 of healthy index was 0.958. The results showed that the non-destructively monitoring method for plug seedlings can provide an effective solution for the monitoring of the plug seedlings.