微酸性电解水对灰葡萄孢菌杀菌效果与作用机制研究

Investigation on Microbicidal Potential and Action Mechanism for Botrytis cinerea of Slightly Acidic Electrolyzed Water

摘要:

研究了微酸性电解水对灰葡萄孢菌悬液纯培养及原位培养的杀菌效果，同时通过扫描电镜和透射电镜初步探究了其对灰葡萄孢菌的杀菌机制。研究表明，随杀菌时间和有效氯质量浓度的增加，微酸性电解水对灰葡萄孢的杀菌能力明显增强；在有效氯质量浓度为10mg/L、杀菌处理15min，以及有效氯质量浓度在30mg/L、杀菌处理10min时，对悬液中灰葡萄孢菌的杀菌率均达99.99%。对用微酸性电解水处理后的灰葡萄孢菌落原位生长状态观察表明，微酸性电解水可以抑制菌落扩生，浓度越高抑生长作用越强，用有效氯质量浓度为30mg/L的微酸性电解水处理菌落3d后，菌丝逐渐变暗黄、枯萎，直至死亡，菌落停止生长，推测微酸性电解水可杀灭灰葡萄孢的新生孢子并抑制菌丝生长。通过扫描及透射电镜观察，发现微酸性电解水处理可导致灰葡萄孢孢子细胞质壁分离，细胞质溢出，细胞器溶解，细胞皱缩，这可能是引起灰葡萄孢菌无法正常生长繁殖，最终达到杀菌效果的原因。

Abstract:

Slightly acidic electrolyzed water is a kind of bactericidal preparation with pH value slightly lower than 7.0 by electrolyzing dilute hydrochloric acid solution or electrolyzing low concentration dilute hydrochloric acid and electrolyte (NaCl/KCl) under nondiaphragm condition. It has a strong ability to kill pathogenic bacteria, but there is little research on the ability and mechanism of killing fungal spores by slightly acidic electrolyzed water. The killing effect of slightly acidic electrolytic water on pure culture spore suspension and in situ culture of Botrytis cinerea was studied, and the germicidal mechanism of water on Botrytis cinerea was preliminarily studied by scanning electron microscopy and transmission electron microscopy. The results showed that the bactericidal ability of slightly acidic electrolyzed water to Botrytis cinerea was increased significantly with the increase of sterilization time and available chlorine concentration (ACC), and bactericidal rate can achieve 99.99% when the available chlorine concentration was not less than 30mg/L and kill time was 10min. The growth state of Botrytis cinerea colony in situ after treatment with slightly acidic electrolytic water showed that slightly acidic electrolytic water could inhibit colony expansion, and the higher the concentration was, the stronger the inhibition effect was. After 3d of treatment with slightly acidic electrolytic water with 30mg/L available chlorine, the mycelium gradually turned yellow and withered until death, and the colony was stopped. It was suggested that microacid electrolyzed water can kill the new spores of Botrytis cinerea and inhibit the growth of mycelia. Through microscope observation of scanning and transmission electron, it was found that treatment with acidic electrolyzed water can make separation of membrane and cell wall, cytoplasmic overflow and abnormal lysis of organelles. It was speculated that these may be the reasons which can achieve the bactericidal effect.