玉米粉中黄曲霉毒素B1微波降解技术

Degradation of Aflatoxin B1 in Corn Flour by Microwave Radiation

摘要:

应用微波辐照技术降解玉米粉中黄曲霉毒素B1(AFB1)，采用免疫亲和柱净化-高效液相色谱-柱后衍生的方法测定玉米粉中AFB1的含量。试验考察了微波辐照功率、微波辐照时间以及AFB1初始含量对其降解效率的影响，并与传统加热法进行比较。结果表明：微波辐照主要利用非热效应对AFB1进行降解，当功率为250 W、时间为30 min时，AFB1降解率大于90%，残留量在国标和欧盟安全限量范围之内。微波辐照处理对玉米粉营养品质影响较小，且AFB1降解产物毒性明显降低，不会产生二次污染。

Abstract:

A microwave irradiation technique was applied for degradation of aflatoxin B1(AFB1) in corn flour, and immunoaffinity column clean-high performance liquid chromatography-post-column derivatization method was applied to determine the content of AFB1. The effects of microwave power, irradiation time and initial quality of AFB1 on degradation efficiency were investigated. Microwave irradiation was compared with conventional heating method. The results showed that the degradation of AFB1 was performed by non-thermal effect. The optimized microwave power was 250 W and irradiation time was 30 min. Under this condition, the degradation rate would reach to 90%. The residues of AFB1 were in the safety limit range of the national standard and the European Union. The nutrition quality of corn flour was slightly affected by the microwave irradiation treatment. The toxicity of AFB1 was reduced obviously and there was no secondary pollution. Therefore, the microwave irradiation can be used as an effective method for the degradation of AFB1. 