脉冲磁场对枯草芽孢杆菌细胞膜流动性的影响

Effects of the Pulsed Magnetic Field on Membrane Fluidity of Bacillus subtilis

摘要:

研究了脉冲磁场对枯草芽孢杆菌细胞膜流动性的影响。以DPH为探针，在 35℃标记45min后采用荧光偏振法测定了枯草芽孢杆菌细胞膜的荧光偏振度和荧光各异向性的变化。结果表明，荧光偏振度和荧光各异向性随着磁场强度和脉冲数的增加而增加，2.0T、2.5T处理25~30个脉冲，3.0T处理 20~30个脉冲，3.3T处理10~30个脉冲时细胞膜的荧光偏振度与对照组相比差异显著（P＜0.05）， 2.0T处理30个脉冲和2.5T处理25~30个脉冲，3.0T处理20~30个脉冲，3.3T处理15~30个脉冲，荧光各异向性与对照组相比差异显著（P＜0.05）。由此表明，脉冲磁场可使枯草芽孢杆菌细胞膜流动性下降，而细胞膜流动性的下降会引起细胞膜功能的丧失，这可能是导致细胞死亡的原因。

Abstract:

Effects of the pulsed magnetic field on membrane fluidity of Bacillus subtilis were investigated. 1,6-diphenyl-1,3,5-hexatriene (DPH) was used as fluorescence probe to label the membrane of B. subtilis at 35℃ for 45min to determine the changes of fluorescence polarization and fluorescence anisotropy of B. subtilis membrane by fluorescence polarization technique. The results indicated that fluorescence polarization and fluorescence anisotropy increased with the increase of intensity and pulse number of PMF. Compared with the control group, fluorescence polarization increased greatly at 2.0T with 25~30 pulses, 2.5T with 25~30 pulses, 3.0T with 20~30 pulses and 3.3T with 10~30 pulses（P＜0.05）. Fluorescence anisotropy also increased greatly at 2.0T with 30 pulses, 2.5T with 25~30 pulses, 3.0T with 20~30 pulses and 3.3T with 15~30 pulses（P＜0.05）. The membrane fluidity decreased, resulting in the lost of the membrane function, when treated by PMF, which suggested a perhaps mechanisms for the cell death.