荷叶活性物质提取工艺与抗氧化活性研究

and Antioxidant Activity of Bioactive Components from Lotus Leaf

摘要:

对荷叶活性物质提取工艺与抗氧化活性进行了研究。结果表明，荷叶抗氧化活性物质最佳提取工艺为提取时间50min、提取温度80℃、乙醇体积分数60%和料液比1∶20；采用生物活性追踪法研究发现，在极性依次增大的正己烷、乙酸乙酯、正丁醇和水相4个极性萃取组分中，乙酸乙酯萃取组分抗氧化活性（总还原力、FRAP法抗氧化能力和DPPH自由基清除能力）最强并具有显著性差异（P<0.001或P<0.01）；通过验证试验发现该组分抗氧化能力（OD593和DPPH自由基清除率）均显著高于阳性对照

关键词:荷叶;抗氧化活性;提取工艺;生物活性追踪法

Abstract:

BHT和GBE（P<0.05）。 The extracting technology and antioxidant activities of lotus leaf were studied. The results showed that the optimal parameters of the extracting technology were the extracting time was 50min, the extracting temperature was 80℃, the concentration of ethanol was 60% and the ratio of sample to extracting solution was 1∶20. The ethyl acetate fraction possessed the strongest antioxidant activities (P<0.001or P<0.01), i. e., the reducing power, FRAP value and DPPH radicals scavenging capacity among the four increasing polar fractions (hexane, ethyl acetate, butanol and aqueous fractions) through the bio-assay guided method. The OD593 values and DPPH radicals scavenging capacities of this fraction were better than those of the positive controls of BHT and GBE (P<0.05) through the experiments of verification. 

Key words:荷叶;抗氧化活性;提取工艺;生物活性追踪法