米根霉菌丝球半连续发酵产乳酸的工艺研

of Lactic Acid by Pellets of Rhizopus oryzae in Semi-continuous Fermentation

摘要:

采用单因素试验和正交试验设计，以乳酸质量浓度及菌丝形态为考察指标，对米根霉首批发酵培养基及补料培养基进行优化，得到适于米根霉半连续发酵的培养基，建立了米根霉半连续发酵工艺，并进行米根霉半连续发酵产乳酸的稳定性试验。得到首批培养基参数为葡萄糖120g/L，(NH4)2SO4 2g/L，NaH2PO4 0.16g/L，KH2PO4 0.14g/L，MgSO4?7H2O 0.2g/L，ZnSO4?7H2O 0.22g/L，CaCO3 60g/L；补料培养基参数为葡萄糖100g/L，（NH4）2SO4 2g/L，KH2PO4 0.1g/L，ZnSO4?7H2O 0.33g/L，MgSO4?7H2O 0.15g/L，CaCO3 50g/L，摇瓶重复稳定发酵30批次，罐发酵重复稳定发酵20批次，葡萄糖转化率最高达到

关键词:乳酸;米根霉;半连续发酵;菌丝球

Abstract:

86％。 By using single-factor test and orthogonal test, the optimized seed medium and feeding medium were obtained, with the aim to study the stability of the production of lactic acid by Rhizopus oryzae though semi-continuous fermentation. The optimization culture medium was as follows: glucose 120g/L，(NH4)2SO4 2g/L，NaH2PO4 0.16g/L，KH2PO4 0.14g/L，MgSO4?7H2O 0.2g/L，ZnSO4?7H2O 0.22g/L，CaCO3 60g/L; the feeding medium was as follows: glucose 100g/L，（NH4）2SO4 2g/L，KH2PO4 0.1g/L，ZnSO4?7H2O 0.33g/L，MgSO4?7H2O 0.15g/L，CaCO3 50g/L. In flask culture 30 repeated stable fermentation were carried out, and 20 batches were carried out in a 5 L stirred tank fermentor. The highest lactic acid yield from glucose was up to 86%.

Key words:乳酸;米根霉;半连续发酵;菌丝球